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            化學交聯結合質譜技術(chemical cross-linking coupled with mass spectrometry,CXMS)技術在近十年內得到了蓬勃發展。被廣泛應用于蛋白質結構鑒定和蛋白質相互作用分析。目前CXMS技術在實際應用存在一定的局限性:1、復雜樣品處理效率偏低;2、能夠提供的交聯信息有限。該技術發展的最大瓶頸已經體現在有限的化學交聯劑選擇和交聯效率上。百靈威作為專業供應商,提供新型化學交聯劑Leiker和d6-Leiker,具有可富集、可切斷、可定量的作用[1]。

       Leiker具有高效的富集功能,比傳統交聯劑有更高的效率

      Figure 1. Leiker(2138035) allowed near 100% enrichment of target peptides from a cross-linked ten-protein mixture diluted with increasing amounts of non-cross-linked E. coli lysates[1].

       Leiker可以顯著提高所能鑒定到的交聯肽段數量

      Figure 2. CXMS analyses of E. coli and C. elegans lysates. The best protein-protein interaction cluster extracted from the Leiker-identified or BS3(146482)-identified (Yang et al., 2012) [2]inter-links from E. coli whole-cell lysates[1].

       Leiker可以進行定量CXMS研究

      Figure 3. Quantitative CXMS analysis of the L7Ae-RNA complex. Reciprocal labeling of RNA-free (F) and RNA-bound (B) L7Ae with [d0]/[d6]-Leiker(2322681)[1].

      References

      [1] Tan D, Li Q, Zhang M J, et al. Trifunctional cross-linker for mapping protein-protein interaction networks and comparing protein conformational states[J]. Elife Sciences, 2016, 5.

      [2] Yang B, Wu Y J, Zhu M, et al. Identification of cross-linked peptides from complex samples.[J]. Nature Methods, 2012, 9(9):904-906.

      新型蛋白質交聯劑

      營銷編號

      產品名稱

      中文名稱

      包裝規格

      2138035

      Leiker, 95%

      賴氨酸特異性可富集型交聯劑

      5 mg
      10 mg

      2322681

      d6-Leiker, 95%

      d6-賴氨酸特異性可富集型交聯劑

      5 mg
      10 mg

      ■ 其他交聯劑系列產品

      產品編號

      產品名稱

      CAS

      包裝規格

      350636

      4-Benzoylbenzoic acid N-succinimidyl ester, 97%

      91990-88-4

      50 mg
      250 mg

      146482

      Bis(sulfosuccinimidyl) suberate sodium salt, BS3, 90%

      82436-77-9

      25 mg
      100 mg

      296329

      Di(N-succinimidyl) 3,3'-dithiodipropionate, DTSP, 97%

      57757-57-0

      1 g
      5 g

      456454

      Di(N-succinimidyl) glutarate, DSG, 97%

      79642-50-5

      50 mg
      250 mg

      253405

      Di(N-succinimidyl) suberate, DSS, 98%

      68528-80-3

      1 g
      5 g

      1410146

      Disuccinimidyl tartrate, DST, 95%

      N/A

      5 mg
      25 mg

      792543

      3,3'-dithiobispropionic acid bis-sulfosuccinimidyl ester, DTSSP, 90%

      81069-02-5

      25 mg
      100 mg

      444282

      N-Succinimidyl iodoacetate, 95%

      39028-27-8

      100 mg
      500 mg

      551313

      Succinimidyl 3-(2-Pyridyldithio)Propionate, SPDP, 97%

      68181-17-9

      100 mg

      相關產品

      產品編號

      英文名稱

      中文名稱

      CAS

      649777

      Acrylamide/?Bis-?acrylamide Solution, 29:1, 40%

      丙烯酰胺/雙丙烯酰胺溶液

      N/A

      398539

      Aprotinin, 6000 U/mg

      抑肽酶

      9087-70-1

      3922801

      BCA Protein Assay Macro Kit

      蛋白質定量試劑盒

      N/A

      266630

      Benzamidine hydrochloride, 99%

      苯甲脒鹽酸鹽

      1670-14-0

      1914018

      CAPS Buffer

      CAPS 緩沖液

      N/A

      327499

      Coomassie Brilliant Blue G

      考馬斯亮藍G

      6104-58-1

      415951

      Dithiothreitol, 99%

      二硫蘇糖醇

      3483-12-3

      1914020

      Phosphate Buffered Saline, 10×

      PBS緩沖液

      N/A

      3910101

      Protease Inhibitor Mix G

      Cocktail蛋白酶抑制劑G

      N/A

      3910201

      Protease Inhibitor Mix M

      Cocktail蛋白酶抑制劑M

      N/A

      1201851

      Protein Color Marker, M.W. 1,200 - 31,000 Da

      蛋白預染Marker

      N/A

      1914019

      SSC Buffer, 20×

      SSC 緩沖液

      N/A

      1913953

      Tris-Glycine-SDS Running Buffer, 10×

      Tris-Glycine-SDS電泳緩沖液

      N/A

      954773

      Trypsin, 2500 units/mg

      胰蛋白酶

      9002-07-7

      其他配套產品

      百靈威高效酶制劑

      百靈威熒光試劑特異性強的染色劑

      百靈威新型生化染色劑
      和染料

      百靈威核心生化試劑

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